Journal: The Journal of International Medical Research

Article Title: Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury

doi: 10.1177/0300060520919592

Figure Lengend Snippet: Primer sequences for real-time quantitative polymerase chain reaction analyses.

Article Snippet: Mouse anti-rat MMP9 (cat. no. NBP2-13173SS) and TIMP2 antibodies (cat. no. NBP1-42375) were purchased from Novus Biologicals Inc. (Littleton, CO, USA).

Techniques: Real-time Polymerase Chain Reaction

Journal: The Journal of International Medical Research

Article Title: Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury

doi: 10.1177/0300060520919592

Figure Lengend Snippet: Lung MMP and TIMP mRNA and protein expression. Changes in MMP2 (a), TIMP2 (b), MMP2/TIMP2 (c), MMP9 (d), TIMP1 (e), and MMP9/TIMP1 (f) in all groups treated with or without LPS. (g) Protein expression in each group as detected by western blotting. LPS 2h, 6h, 12h, and 24h indicate 2, 6, 12, and 24 hours after LPS injection in the acute lung injury group, respectively. Data are shown as mean ± standard deviation (n = 6 per group). * P < 0.05 vs. control, # P < 0.05 vs. LPS 2h. Control, normal control group; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; LPS, lipopolysaccharide

Article Snippet: Mouse anti-rat MMP9 (cat. no. NBP2-13173SS) and TIMP2 antibodies (cat. no. NBP1-42375) were purchased from Novus Biologicals Inc. (Littleton, CO, USA).

Techniques: Expressing, Western Blot, Injection, Standard Deviation, Control

Journal: Disease markers

Article Title: Combination of Strong MMP-2 and Weak TIMP-2 Immunostainings Is a Significant Prognostic Factor in Endometrial Carcinoma

doi: 10.1155/2013/416870

Figure Lengend Snippet: The independent and significant prognostic factors for the cancer-specific survival of endometrial cancer patients as evaluated by the Cox regression method. Relative risk of death, its 95% confidence interval, and P values are given for each covariate. n = 225.

Article Snippet: A mouse monoclonal antibody to MMP-2 (CA-4001, Diabor Ltd., Oulu, Finland) or TIMP-2 (MAB 971, R&D Systems, Minneapolis, MN, USA) was used as the primary antibody.

Techniques:

Journal: JCI Insight

Article Title: Cannabinoid enhancement of lncRNA MMP25-AS1 / MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection

doi: 10.1172/jci.insight.167903

Figure Lengend Snippet: Whole colon tissue sections were immunostained for MMP25 ( A ) (green) and TIMP2 ( E ) (red) and DAPI for nuclear staining (blue). Note the increased MMP25 ( A ) and decreased TIMP2 ( E ) protein expression, specifically in CE of VEH/SIV ( n = 5) compared with THC/SIV ( n = 5) and control ( n = 5) RMs. Representative immunofluorescence images were captured using a Zeiss confocal microscope at 20× original magnification. Scale bars: 50 μm. The demarcation of CE regions excluding the lamina propria compartment using the freehand tool in HALO ( B ). Differences in MMP25 ( C ) and TIMP2 ( F ) signal intensity between groups were analyzed using 1-way ANOVA employing the Prism v9 (GraphPad Prism). A P value of <0.05 was considered significant. Data represent mean ± SEM. STRING v 11.0 software was used to predict the MMP25 protein-protein interactions with other proteins ( D ). Each node represents all the proteins produced by a single protein-coding gene locus. Edges represent protein-protein associations.

Article Snippet: Immunofluorescence studies for the detection of MMP25 (1:50) (Abcam; ab56309), TIMP2 (1:100) (Novus Biologicals; NBP2-01573), CD47 (1:200) (Mybiosource.com; MBS8106360), MPO (1:6,000) (Abcam; A0398), and CD11b (1:100) (Abcam; ab34216) were performed as described previously ( ).

Techniques: Staining, Expressing, Control, Immunofluorescence, Microscopy, Software, Protein-Protein interactions, Produced

Journal: JCI Insight

Article Title: Cannabinoid enhancement of lncRNA MMP25-AS1 / MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection

doi: 10.1172/jci.insight.167903

Figure Lengend Snippet: At the transcriptional level, MMP25-AS1 directly interacts with MMP25 , a matrix metalloproteinase known to enhance the activation of neutrophils and promote inflammation and barrier disruption, allowing microbial translocation. Increased TIMP2 expression results in a further decrease of MMP25 expression at the protein level. The reduction of MMP25 expression also results in a concomitant decrease in CXCL5 and CCL15 expression, 2 potent neutrophil chemoattractants activated by MMP25-mediated cleavage, thus highlighting THC’s potential to block a feed-forward mechanism for neutrophil recruitment. Importantly, THC administration to SIV-infected RMs on long-term cART successfully reduced MMP25 expression in JE of chronically SIV-infected RMs, while concurrently preserving the expression of its natural antisense lncRNA regulator MMP25-AS1. From a therapeutic standpoint, long-term low-dose THC administered as an adjunct to cART represents a potentially safe and effective intervention to dampen chronic immune activation and inflammation, a major driver of HIV-associated comorbidities in virally suppressed PLWH.

Article Snippet: Immunofluorescence studies for the detection of MMP25 (1:50) (Abcam; ab56309), TIMP2 (1:100) (Novus Biologicals; NBP2-01573), CD47 (1:200) (Mybiosource.com; MBS8106360), MPO (1:6,000) (Abcam; A0398), and CD11b (1:100) (Abcam; ab34216) were performed as described previously ( ).

Techniques: Activation Assay, Disruption, Translocation Assay, Expressing, Blocking Assay, Infection, Preserving